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non-essential amino acids (neaa)  (Mediatech)

 
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    Mediatech non-essential amino acids (neaa)
    Non Essential Amino Acids (Neaa), supplied by Mediatech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/non-essential amino acids (neaa)/product/Mediatech
    Average 90 stars, based on 1 article reviews
    non-essential amino acids (neaa) - by Bioz Stars, 2026-03
    90/100 stars

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    GLS1-mediated glutamine metabolism maintains cell proliferation by providing proliferative response substrates. ( A ) Representative bright-field images of liver organoids before (upper panel) and after (lowing panel) the treatment with or without CB839 and <t>NEAA</t> for 48 h (Bar = 1000 μm) and the quantification of organoid size ( B ). ( C ) Cell viability measurement was performed using presto blue. Relative cell viability was calculated by normalizing to Control after 48 h of incubation ( n = 5). ( D ) Protein synthesis in liver organoids treated with or without CB839 and NEAA for 48 h was determined by L-homopropargylglycine (HPG) mean fluorescence intensity (MFI), ( n = 5). (E) Proliferation of liver organoids treated with or without CB839 and NEAA for 3 h was determined by BrdU incorporation, ( n = 5). All data are means ± SEM. ∗ P < 0.05, ∗∗ P < 0.01, one-way ANOVA was used
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    GLS1-mediated glutamine metabolism maintains cell proliferation by providing proliferative response substrates. ( A ) Representative bright-field images of liver organoids before (upper panel) and after (lowing panel) the treatment with or without CB839 and <t>NEAA</t> for 48 h (Bar = 1000 μm) and the quantification of organoid size ( B ). ( C ) Cell viability measurement was performed using presto blue. Relative cell viability was calculated by normalizing to Control after 48 h of incubation ( n = 5). ( D ) Protein synthesis in liver organoids treated with or without CB839 and NEAA for 48 h was determined by L-homopropargylglycine (HPG) mean fluorescence intensity (MFI), ( n = 5). (E) Proliferation of liver organoids treated with or without CB839 and NEAA for 3 h was determined by BrdU incorporation, ( n = 5). All data are means ± SEM. ∗ P < 0.05, ∗∗ P < 0.01, one-way ANOVA was used
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    GLS1-mediated glutamine metabolism maintains cell proliferation by providing proliferative response substrates. ( A ) Representative bright-field images of liver organoids before (upper panel) and after (lowing panel) the treatment with or without CB839 and NEAA for 48 h (Bar = 1000 μm) and the quantification of organoid size ( B ). ( C ) Cell viability measurement was performed using presto blue. Relative cell viability was calculated by normalizing to Control after 48 h of incubation ( n = 5). ( D ) Protein synthesis in liver organoids treated with or without CB839 and NEAA for 48 h was determined by L-homopropargylglycine (HPG) mean fluorescence intensity (MFI), ( n = 5). (E) Proliferation of liver organoids treated with or without CB839 and NEAA for 3 h was determined by BrdU incorporation, ( n = 5). All data are means ± SEM. ∗ P < 0.05, ∗∗ P < 0.01, one-way ANOVA was used

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: The metabolic shift of glutaminase 2 to glutaminase 1 promotes LGR5 + progenitor cell proliferation in liver cirrhosis

    doi: 10.1007/s00018-025-05772-z

    Figure Lengend Snippet: GLS1-mediated glutamine metabolism maintains cell proliferation by providing proliferative response substrates. ( A ) Representative bright-field images of liver organoids before (upper panel) and after (lowing panel) the treatment with or without CB839 and NEAA for 48 h (Bar = 1000 μm) and the quantification of organoid size ( B ). ( C ) Cell viability measurement was performed using presto blue. Relative cell viability was calculated by normalizing to Control after 48 h of incubation ( n = 5). ( D ) Protein synthesis in liver organoids treated with or without CB839 and NEAA for 48 h was determined by L-homopropargylglycine (HPG) mean fluorescence intensity (MFI), ( n = 5). (E) Proliferation of liver organoids treated with or without CB839 and NEAA for 3 h was determined by BrdU incorporation, ( n = 5). All data are means ± SEM. ∗ P < 0.05, ∗∗ P < 0.01, one-way ANOVA was used

    Article Snippet: Rescue experiments were performed with 5 mM glutathione reduced ethyl ester (GSH-EE), 1x MEM Non-Essential Amino Acids solution (NEAA) from Thermo Fisher Scientific.

    Techniques: Control, Incubation, Fluorescence, BrdU Incorporation Assay

    Schematic model illustrating the effect of the swift of GLS2 to GLS1 on ROS-Wnt/β-catenin signaling and proliferation in liver organoids. GLS1 reduces the generation of ROS, promotes nuclear translocation of β-Catenin and then promotes Wnt/β-Catenin target genes transcription in liver organoids. In addition, GLS1 accelerates the metabolism of Glutamine and provides proliferative response substrates such as amino acids and nucleotides for cell proliferation. Abbreviations: Gln, Glutamine; GSH, Glutathione; Glu, Glutamate; ROS, Reactive oxygen species; NEAA: non-essential amino acids

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: The metabolic shift of glutaminase 2 to glutaminase 1 promotes LGR5 + progenitor cell proliferation in liver cirrhosis

    doi: 10.1007/s00018-025-05772-z

    Figure Lengend Snippet: Schematic model illustrating the effect of the swift of GLS2 to GLS1 on ROS-Wnt/β-catenin signaling and proliferation in liver organoids. GLS1 reduces the generation of ROS, promotes nuclear translocation of β-Catenin and then promotes Wnt/β-Catenin target genes transcription in liver organoids. In addition, GLS1 accelerates the metabolism of Glutamine and provides proliferative response substrates such as amino acids and nucleotides for cell proliferation. Abbreviations: Gln, Glutamine; GSH, Glutathione; Glu, Glutamate; ROS, Reactive oxygen species; NEAA: non-essential amino acids

    Article Snippet: Rescue experiments were performed with 5 mM glutathione reduced ethyl ester (GSH-EE), 1x MEM Non-Essential Amino Acids solution (NEAA) from Thermo Fisher Scientific.

    Techniques: Translocation Assay